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KMID : 0811720180220020145
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Korean Journal of Physiology & Pharmacology
2018 Volume.22 No. 2 p.145 ~ p.153
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Toll-like receptor 2 promotes neurogenesis from the dentate gyrus after photothrombotic cerebral ischemia in mice
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Seong Kyung-Joo
Kim Hyeong-Jun
Cai Bangrong
Kook Min-Suk
Jung Ji-Yeon
Kim Won-Jae
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Abstract
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The subgranular zone (SGZ) of hippocampal dentate gyrus (HDG) is a primary site of adult neurogenesis. Toll-like receptors (TLRs), are involved in neural system development of Drosophila and innate immune response of mammals. TLR2 is expressed abundantly in neurogenic niches such as adult mammalian hippocam-pus. It regulates adult hippocampal neurogenesis. However, the role of TLR2 in adult neurogenesis is not well studied in global or focal cerebral ischemia. Therefore, this study aimed to investigate the role of TLR2 in adult neurogenesis after photochemi-cally induced cerebral ischemia. At 7 days after photothrombotic ischemic injury, the number of bromodeoxyuridine (BrdU)-positive cells was increased in both TLR2 knock-out (KO) mice and wild-type (WT) mice. However, the increment rate of BrdU-positive cells was lower in TLR2 KO mice compared to that in WT mice. The number of doublecortin (DCX) and neuronal nuclei (NeuN)-positive cells in HDG was decreased after photothrombotic ischemia in TLR2 KO mice compared to that in WT mice. The survival rate of cells in HDG was decreased in TLR2 KO mice compared to that in WT mice. In contrast, the number of cleaved-caspase 3 (apoptotic marker) and the num-ber of GFAP (glia marker)/BrdU double-positive cells in TLR2 KO mice were higher than that in WT mice. These results suggest that TLR2 can promote adult neurogene-sis from neural stem cell of hippocampal dentate gyrus through increasing prolifera-tion, differentiation, and survival from neural stem cells after ischemic injury of the brain.
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KEYWORD
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Hippocampus, Ischemia, Neural stem cell, Neurogenesis, Toll-like receptor 2
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